Cardiac injury in mammals and amphibians typically leads to scarring, with minimal regeneration of heart muscle. Department of cell biology, department of cardiology, howard hughes medical institute, harvard medical school, childrens hospital, 320 longwood avenue, boston, ma 02115, usa. In contrast to humans and other mammals, zebrafish hearts regenerate after substantial injury or tissue damage. The role of the ecm during zebrafish heart regeneration has. Clustering results were visualized using javatreeview manual. Macrophages directly contribute collagen to scar formation. Proteomics analysis of extracellular matrix remodeling during. Collectively, our study identifies h3k27me3mediated silencing of structural genes as requisite for zebrafish heart regeneration and suggests that repression of similar structural components in the border zone of an infarcted human heart might improve its regenerative capacity. Complete regeneration of the zebrafish heart is driven by the strong proliferation response of. Article pdf available in nature 4987455 june 20 with.
Autophagic vesicle formation in the heart ventricle is increased during zebrafish cardiac regeneration. The growing interest in zebrafish research was paralleled by an increase in tools and methods. The zebrafish as a model for complex tissue regeneration. Pdf in vivo cardiac reprogramming contributes to zebrafish. Telomerase is essential for zebrafish heart regeneration. Autophagy activation in zebrafish heart regeneration scientific. Zebrafish heart regeneration as a model for cardiac tissue repair. In vivo cardiac reprogramming contributes to zebrafish heart regeneration.
Zebrafish heart regeneration as a model for cardiac tissue. Regeneration of ventricular myocardium in the resected zebrafish heart. In contrast, zebrafish hearts regenerate even when up to 20% of the ventricle is amputated. There are currently several injury models that stimulate heart regeneration in zebrafish, including surgical resection of the ventricular apex, the first and mostused injury method, cryoinjury, and inducible genetic ablation 52 56. Our data indicate that zebrafish heart regeneration is associated with.
The zebrafish heart regenerates after cryoinjuryinduced myocardial. Clustering results were visualized using javatreeview 42. Zebrafish are able to efficiently regenerate lost cardiac muscle, and is used as a model to understand why natural heart regeneration is blocked in mammals. Currently, the research on heart regeneration in newts and zebrafish is based. Zebrafish as a smart model to understand regeneration after heart. Heart regeneration in the mexican cavefish cell press. Gene expression analysis of zebrafish heart regeneration plos. Zebrafish are able to replace lost heart muscle efficiently, and are used as a model to understand why natural heart regeneration after a heart attack, for instance is blocked in mammals. Adult sox10derived cardiomyocytes contribute to zebrafish heart. Myocardial infarction mi in humans is a common cause of cardiac injury and results in irreversible loss of myocardial cells and formation of. Review getting to the heart of regeneration in zebra. Scientists describe the mechanism of heart regeneration in the zebrafish. The role of the epicardium during zebrafish heart regeneration recent results have shed light on the role of the outer nonmuscle layer of the heart, the epicardium, in cardiac regeneration. Zebrafish, which any of you who are fish fans here, keep.
In vivo cardiac reprogramming contributes to zebrafish heart regeneration article pdf available in nature 4987455 june 20 with 315 reads how we measure reads. Zebrafish heart regeneration occurs by cardiomyocyte. Bony fish like the tiny zebrafish have a remarkable ability that mammals can only dream of. Recent studies have revealed that macrophages are essential players in the regeneration of the salamander limb 12, adult zebrafish tail fin 16 and neonatal mouse heart 10.
The study of zebrafish as a leading model organism for developmental biology is rapidly expanding. Zebrafish heart regeneration proceeds through injuryinduced proliferation of. Scientists describe the mechanism of heart regeneration in. After rippling and manual rearrangement to maximize order lod score and minimize length, one lg. Primary contribution to zebrafish heart regeneration by. Department of cell biology, duke university medical center, durham, nc 27710, united states available online 24 november 2006 abstract a scienti. Zebrafish, which any of you who are fish fans here, keep in the fish tank, can get to be about two inches long. During embryogenesis, the epicardium migrates out as a sheet from the proepicardium, a cluster or mesodermderived cells near the liver primordium and the septum transversum, to envelop the developing myocardial tube 43.
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